Weislab.wikia.com/transformationbacterial

Since the proper transformation procedure is dependent on the cells used, consult the .pdf resources on the Competent cells page. This generic protocol usually works and can be used as a starting point.
 * 1) Thaw competent cells on ice
 * 2) Add 0.5 ul of plasmid to cells and tap gently to mix
 * 3) Incubate on ice for 20 minutes
 * 4) Heat shock at 42C for 45 seconds
 * 5) Back on ice for 2 minutes
 * 6) Add 200-1,000 ul LB and incubate at 37C for 5 minutes (AMP selection) or 1 hour (KAN selection)
 * 7) Plate cells on one or more plates based on expected efficiency
 * 8) Rejoice.