YPD + G418 plates

YPD + G418 plates

To make these plates you'll need yeast extract, peptone, agar, and G418. All of these can be found by the balance, except for the G418 which is found in Freezer 1.

To make 500mL of YPD + 1xG418 media,


 * 1) Pour 450mL of miliQ-H2O into a 500mL glass bottle with a stir bar.
 * 2) Add 5g of yeast, 10 g of peptone, and 10g of Agar.
 * 3) Stir well until dissolved.
 * 4) Autoclave this with 25 minites of sterilization time and 0 min of dry time.
 * 5) Let the bottle cool for a bit and then add 500uL of 1000x G418, and 50mL of 20% Dextrose
 * 6) Let these mix for abit
 * 7) Pour the media onto sterile empty plates (about 25mL per plate)
 * 8) Let the plates cool and then store in a fridge