Yeast plates

Common yeast plates
Many yeast plates are kept as common lab stocks. It is Brett's job to replenish them. It is your responsibility to make plates that are not commonly ordered. Yeast nitrogen base, agar and peptone are kept near the table top centrifuge while amino acid dropout solutions are kept in the 4C fridge by Evgeny's bench (besides -URA 100X solution which is kept on the chemical shelves to avoid precipitation).

Plates ordered from the plate pouring facility in Koshland show much contamination after a couple months. We are considering alternatives including storing our stocks in the cold room to ameliorate this issue.

You can obtain the forms needed for ordering plates from the Koshland (LL41) facility from Brett. Print them out and put them in the basket of LL41 and put the 10X dropout media into the small fridge in their facility. Make sure to turn back on the UV lights after leaving.

Common yeast plates supplied by Ryan include:

YPD

Minimal (YNB only, no amino acids)

SC - Leucine

SC - Uracil

SC - Histidine

SC - Tryptophan

Plates you must make yourself include: 

YPD + G418 plates

Multiple amino acid drop-out plates

etc.