Reagents

This is a list of common reagents. Follow the link for additional info.

Ammonium persulfate (10% APS)

Ampicillin

BL21 (DE3) (RIL) expression bacteria

Beta-mercaptoethanol

Chloramphenicol

Competent cells

dNTPs

G418

Kanamycin

Phenol:chloroform:isoamyl alcohol (25:24:1)

Platinum Pfx (DNA Polymerase)

Primer ordering

Resolving buffer (4X)

Stacking buffer (4X)

TEMED

Yeast plates