DNA Extraction (Smash N Grab DNA Prep)

 DNA Extraction Protocol

 Day 1:
 * 1)  Grow yeast in 2mL of YPD overnight. (   Doesn't matter how dense it gets   )
 * 2)  Prepare 100%EtOH and 70%EtOH and store in freezer.
 *  100% EtOH can be found under the hood.

 Day 2:
 * 1)  Pellet 1.5mL @8,000rpm for 2min
 * 2)  Resuspend in 400uL of Lysis Buffer vortex
 *  Elisa has Lysis Buffer. To make it use other lab persons stocks to mix the following. All should be sterile.

 ADD THIS UNDER THE HOOD.
 *  2% Triten X-100
 *  1% SDS
 *  100uM NaCl
 *  10mM TrisHCl pH 8.0
 *  1mM EDTA pH 8.0
 * 1)  Add about ~200uL of glass beads (   Doesn't have to be exact, just as least as possible   )
 * <p style="margin-bottom: 0in"> Glass beads are by the velvets
 * 1) <p style="margin-bottom: 0in"> Add 400 uL of phenol/chloroform/isoamyl alcohol pH 8.0
 * <p style="margin-bottom: 0in"> This is found in fridge 1 in a brown bottle mixture.

<p style="margin-bottom: 0in"> When adding, don't forget to penetrate water layer to reach phenol solution!
 * 1) <p style="margin-bottom: 0in"> Secure caps, cover with aluminum foil, and vortex the centrifuge tubes for 2min.
 * <p style="margin-bottom: 0in"> The vortex is under the hood.
 * <p style="margin-bottom: 0in"> MAKE SURE IT'S SECURE!
 * 1) <p style="margin-bottom: 0in">Centrifuge @ 14,000rpm for 5min.
 * 2) <p style="margin-bottom: 0in">Transfer top aqueous layer to a new tube using a pipette.
 * <p style="margin-bottom: 0in">Be careful not to pull up anything from the middle layer! It's better to loose some of the aqueous layer than to contaminate it!
 * <p style="margin-bottom: 0in">Top layer is DNA, middle layer is yeast lipids, bottom layer is glass beads.
 * <p style="margin-bottom: 0in">Trash the rest in the solid waste and liquid waste containers under the hood!
 * 1) <p style="margin-bottom: 0in">Add 1/10Xs volume of 3M NaAcetate pH 7.0 (~40uL) and 2.5Xs volume of 100% EtOH @ -20degrees Celsius (~1,000uL)
 * <p style="margin-bottom: 0in">The NaAcetate can be found with Elisa
 * <p style="margin-bottom: 0in">The EtOH can be found under the hood in a paint thinner like bottle labeled alcohol. You should store it in a freezer the night before.
 * 1) <p style="margin-bottom: 0in"> Mix this well in a normal vortex
 * 2) <p style="margin-bottom: 0in"> Incubate this for `10min @-20degrees Celsius in a water bath.
 * 3) <p style="margin-bottom: 0in"> Centrifuge @ 14,000rpm for 15min.
 * 4) <p style="margin-bottom: 0in"> Remove supernatant.
 * 5) <p style="margin-bottom: 0in"> Add 500uL of 70% -20degrees Celsius EtOH
 * <p style="margin-bottom: 0in"> Do not resuspend pellet just add and invert a few times
 * <p style="margin-bottom: 0in"> You'll have to dilute the 100%EtOH and store cool it in the freezer for a few hours.
 * 1) <p style="margin-bottom: 0in"> Centrifuge @ 14,000rpm for 5min
 * 2) <p style="margin-bottom: 0in"> Remove EtOH and let the pellet air dry for 30min with paper towel or OVERNIGHT
 * 3) <p style="margin-bottom: 0in"> Resuspend in 50uL of ddH20.
 * <p style="margin-bottom: 0in"> DO NOT VORTEX
 * 1) <p style="margin-bottom: 0in"> You can now use 1uL of this for the PCR