Bradford Assay

Bradford assays are used to determine the concentration of protein within a sample.

It involves mixing some of your sample with a dye reagent made by Bio-rad and measuring the absorbance at 595 nm on a spectrophotometer. Typically, absorbance is used to calculate concentration in mg/ml against BSA standards in the 0.1 to 1.0 mg/ml range. Although the assay is often performed in microfuge tubes/cuvettes, it can be performed with 96-well microplates and a plate reader for many samples.

The Bio-Rad dye reagent is located in the door of 4C Fridge. See the product manual for more details.